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Related Experiment Videos

Basic fibroblast growth factor expression precedes flow-induced arterial enlargement

T M Singh1, K Y Abe, T Sasaki

  • 1Department of Vascular Surgery, Stanford University, Stanford, California, 94305, USA.

The Journal of Surgical Research
|September 12, 1998
PubMed
Summary

Basic fibroblast growth factor (bFGF) is upregulated in arteries experiencing increased blood flow, particularly in smooth muscle cells, suggesting its role in flow-induced arterial enlargement and adaptive remodeling.

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Area of Science:

  • Vascular Biology
  • Molecular Biology
  • Cardiovascular Physiology

Background:

  • Arterial enlargement is a response to increased blood flow, but the underlying molecular mechanisms remain unclear.
  • Basic fibroblast growth factor (bFGF) is a known mitogen for endothelial cells (EC) and smooth muscle cells (SMC), influencing proliferation and differentiation.

Purpose of the Study:

  • To investigate whether basic fibroblast growth factor (bFGF) expression increases in arteries subjected to elevated blood flow in vivo.
  • To determine the cellular localization and temporal expression patterns of bFGF in response to increased arterial flow.

Main Methods:

  • Carotid-jugular arteriovenous fistulas (AVF) were created in rabbits to induce increased carotid artery blood flow.
  • Arteries were analyzed at 1, 3, and 7 days post-AVF creation using RT-PCR, immunohistochemistry, and Western blotting.

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  • Transmission immunoelectron microscopy was used to confirm bFGF subcellular localization.

    • Main Results:

    • Arterial blood flow and wall shear stress significantly increased in AVF animals, leading to arterial enlargement by day 7.
    • bFGF mRNA transcription was detected early (1-3 days) and increased by day 7.
    • bFGF protein was localized in ECs under normal and high flow, while SMC and adventitia showed increased bFGF expression starting at day 1 and peaking by day 7.
    • Nuclear localization of bFGF was confirmed in SMCs.

    Conclusions:

    • Smooth muscle cell expression of bFGF is induced by high flow conditions and precedes arterial enlargement.
    • Basic fibroblast growth factor (bFGF) likely plays a crucial role in the adaptive remodeling of arteries in response to increased blood flow.