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Related Experiment Videos

Lysosome membrane permeability to anions

A R Klemm1, K L Pell, L M Anderson

  • 1Division of Developmental Biology, Jefferson Medical College and Nemours Research Programs, Alfred I. duPont Hospital for Children, P. O. Box 269, Wilmington, DE 19899, USA.

Biochimica Et Biophysica Acta
|September 12, 1998
PubMed
Summary

Rat liver lysosome permeability to anions was studied. Results suggest anion entry depends on factors like charge and molecular structure, not just specific transporters.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Membrane Transport

Background:

  • Lysosomes are key organelles for cellular degradation.
  • Understanding lysosomal permeability is crucial for drug delivery and disease research.
  • Specific transporters regulate lysosomal content, but non-specific permeation also occurs.

Purpose of the Study:

  • To investigate the permeability of rat liver lysosomes to various inorganic and aliphatic organic anions.
  • To determine the relationship between anion structure and lysosomal membrane permeation.
  • To explore the mechanisms underlying anion transport across the lysosomal membrane.

Main Methods:

  • Lysosomes were isolated from rat liver.
  • An osmotic-protection methodology was employed using lysosomal hexosaminidase latency as a marker.

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  • Lysosomes were incubated in solutions containing potassium salts of different anions at 25°C with valinomycin.

    • Main Results:

    • Anion permeance decreased in the order: thiocyanate, nitrate, iodide > bromide > chloride > sulfate.
    • This order did not align with known anion transporters but resembled the lyotropic series.
    • For organic acids, permeation correlated with the undissociated acid's pKa, suggesting a role for both undissociated and anionic forms.

    Conclusions:

    • Lysosomal permeability to anions is influenced by factors beyond specific transporter affinity, including charge and molecular properties.
    • The lyotropic series may provide a better model for inorganic anion permeation than known transporters.
    • Both undissociated organic acids and their anionic forms can permeate the lysosomal membrane.