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A new subculture and nematocidal assay using a species of diplogastridae
J Yanagida1, R Matsuhashi, I Watanabe
1School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Chemical & Pharmaceutical Bulletin
|September 12, 1998
Abstract:
A new subculture method and a novel microplate assay for nematocidal activity using a species of Diplogastridae have been developed. The assay gives results rapidly, with high sensitivity in 4 h, and indicated good correlation between action mechanism and the nematode shape when examining 15 known compounds, including the antiparasitic avermectin, antimalarial quinine, and the gamma-amino-n-butyric acidA (GABAA) activated Cl- channel antagonist picrotoxinin. Thus new assay could be used as a primary screening method for new nematocidal compounds.