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Elimination of inter-species reactive anti-IgG antibodies by affinity chromatography

M E Johansson, J A Espmark

    Journal of Immunological Methods
    |January 1, 1978
    PubMed

    Inter-species reactive anti-IgG antibodies are disturbing in blocking and interference experiments with the sensitive mixed hemadsorption (MH) technique. By affinity chromatography this disturbance could be efficiently eliminated. Anti-IgG sera were passed through columns containing heterologous IgG. The absorption technique is rapid and simple. After one absorption the inter-species reactivity in anti-IgG sera had been decreased thousand-fold, while any loss of species-specific reactivity could not be detected. Such absorbed anti-IgG sera reacted only with homologous antibodies. The effect is exemplified with e.g. blocking of human HLA antibodies by anti-beta2-microglobulin from rabbits.

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