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Related Experiment Videos

The repeatability of tear mucus ferning grading

C D Pensyl1, S M Dillehay

  • 1University of Alabama at Birmingham School of Optometry, Department of Optometry, 35294-0010, USA.

Optometry and Vision Science : Official Publication of the American Academy of Optometry
|September 12, 1998
PubMed
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Rolando's tear ferning grading system shows high repeatability for classifying normal versus abnormal tear patterns. This subjective method is reliable for diagnosing conditions like keratoconjunctivitis sicca.

Area of Science:

  • Ophthalmology
  • Tear film analysis
  • Diagnostic methods

Background:

  • Tear mucus ferning patterns are graded subjectively by Rolando's system.
  • Grades 1 and 2 are normal, while grades 3 and 4 suggest keratoconjunctivitis sicca.

Purpose of the Study:

  • To assess the intraobserver and interobserver repeatability of Rolando's tear ferning classification system.

Main Methods:

  • 418 photographic slides of tear ferning patterns were graded by two independent investigators.
  • One investigator graded the slides twice under masked conditions.
  • The other investigator graded the slides once.

Main Results:

  • High intraobserver agreement (85.41%) and interobserver agreement (80.62%–86.12%) were found for the four-grade system.

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  • Classifying normal (grades 1-2) versus abnormal (grades 3-4) patterns showed excellent repeatability.
  • Intraobserver repeatability for normal/abnormal classification was 94.50%, with interobserver agreement at 92.10%–94.26%.
  • Conclusions:

    • Rolando's grading system demonstrates high agreement in both intraobserver and interobserver trials.
    • The system is considered an easy and consistent method for classifying tear ferning patterns.
    • This supports its utility in diagnosing dry eye disease.