Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualization and quantitation of iontophoretic pathways using confocal microscopy

N G Turner1, R H Guy

  • 1Department of Biopharmaceutical Sciences, University of California, San Francisco, USA.

The Journal of Investigative Dermatology. Symposium Proceedings
|September 12, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and validation of a minimally invasive and image-guided tape stripping method to sample atopic skin in children.

Clinical and experimental dermatology·2023
Same author

Assessment of Drug Delivery Kinetics to Epidermal Targets In Vivo.

The AAPS journal·2021
Same author

Stratum Corneum Sampling to Assess Bioequivalence between Topical Acyclovir Products.

Pharmaceutical research·2019
Same author

Comparison of patient (POEM), observer (EASI, SASSAD, TIS) and corneometry measures of emollient effectiveness in children with eczema: findings from the COMET feasibility trial.

The British journal of dermatology·2018
Same author

Topical bioavailability of diclofenac from locally-acting, dermatological formulations.

International journal of pharmaceutics·2017
Same author

In situ detection of salicylate in Ocimum basilicum plant leaves via reverse iontophoresis.

Chemical communications (Cambridge, England)·2015

Laser scanning confocal microscopy visualized how iontophoresis enhances drug delivery through hairless mouse skin. Follicular pathways are key for deep penetration, complementing non-follicular transport.

Area of Science:

  • Dermatology and Pharmaceutical Sciences
  • Biophysical Chemistry
  • Optical Imaging Techniques

Background:

  • Understanding skin penetration is crucial for effective transdermal drug delivery.
  • Iontophoresis offers a method to enhance drug transport across the skin barrier.
  • Limitations in visualizing and quantifying transport pathways hinder optimization.

Purpose of the Study:

  • To visualize and quantify the penetration of a model compound (calcein) in hairless mouse skin using iontophoresis.
  • To evaluate the contribution of follicular and non-follicular pathways to iontophoretic transport.
  • To develop and apply advanced imaging strategies for accurate depth-dependent analysis.

Main Methods:

  • Utilized laser scanning confocal microscopy (LSCM) for optical sectioning of unfixed skin.

Related Experiment Videos

  • Employed a model anionic fluorescent compound (calcein) as the permeant.
  • Integrated standard in vitro diffusion cell methodology and fluorometric assays for flux measurements.

    • Main Results:

    • LSCM successfully visualized calcein penetration, revealing enhanced delivery via follicular structures to significant depths.
    • Non-follicular transport was observed, particularly at superficial skin levels.
    • Quantitative analysis indicated that follicular pathways, despite smaller surface area, are highly efficient for iontophoretic delivery.

    Conclusions:

    • Iontophoresis significantly enhances the delivery of calcein into and across hairless mouse skin.
    • Follicular transport is a major contributor to iontophoretic drug delivery efficiency.
    • This study advances the comprehension of iontophoretic mechanisms for transdermal drug delivery.