Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A method for assessing strand breaks in DNA

L Peng1, M J Brisco, A A Morley

  • 1Department of Hematology, Flinders University of South Australia, Bedford Park, South Australia, 5042, Australia.

Analytical Biochemistry
|September 15, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Role of exogenous supplementation of umbilical cord blood immune cells in reconstructing immune function and restoring hematopoietic function in elderly high-risk myeloid neoplasms patients].

Zhonghua yi xue za zhi·2026
Same author

HED-Derived iPSCs Reveal Neurofunctional Defects in Ectodermal Dysplasia.

Journal of dental research·2026
Same author

A review of established and new developments in local therapies for liver cancer.

ESMO gastrointestinal oncology·2026
Same author

[Radiofrequency catheter ablation of atrioventricular reentrant tachycardia originating from the middle cardiac vein and small cardiac vein in 4 children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

[Mutation characteristics and prognosis of patients with Fanconi anemia signaling pathway gene mutation myeloproliferative neoplasm].

Zhonghua yi xue za zhi·2026
Same author

[Application of a novel intestinal diversion stent in the treatment of mid-low rectal anastomotic leakage].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2026
Same journal

The Long Run: A Tribute to Arthur Joseph Lawrence Cooper.

Analytical biochemistry·2026
Same journal

Evaluation of a method for affinity measurement using solution equilibrium titration with magnetic beads.

Analytical biochemistry·2026
Same journal

Metabolomics approach using UHPLC/QE-MS for the mechanism of He Xue Ming Mu tablets on non-proliferative diabetic retinopathy.

Analytical biochemistry·2026
Same journal

UniRES-GO: Unified residue-level early fusion of sequence and predicted structure for protein function prediction.

Analytical biochemistry·2026
Same journal

IgG detection by enzyme-linked mass spectrometric assay versus color, fluorescent, ECL in buffer and serum.

Analytical biochemistry·2026
Same journal

A PCR-based assay for distinguishing between 293, 293T, and 293E cell lines.

Analytical biochemistry·2026
See all related articles

A new TDT assay offers a simple method to detect DNA strand breaks. This DNA damage assay is more sensitive than gel electrophoresis and correlates well with PCR-based methods.

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • DNA strand breaks are critical indicators of DNA damage and cellular apoptosis.
  • Existing methods for detecting DNA strand breaks, such as gel electrophoresis and PCR-based assays, can be laborious or lack sensitivity.

Purpose of the Study:

  • To develop a simple, sensitive, and semiquantitative method for assessing DNA strand breaks.
  • To compare the efficacy of the new method against established techniques.

Main Methods:

  • Development of a DNA strand break assay utilizing terminal deoxynucleotidyl transferase (TDT).
  • The TDT assay incorporates labeled deoxycytidine triphosphate (dCTP) and dideoxy-CTP (ddCTP) to ensure reaction completion.
  • Validation of the TDT assay on 21 blood or bone marrow samples with varying DNA degradation levels, comparing results with gel electrophoresis and a PCR-based N-ras assay.

Related Experiment Videos

Main Results:

  • The TDT assay demonstrated higher sensitivity in detecting DNA strand breaks compared to gel electrophoresis.
  • A strong correlation was observed between the results obtained from the TDT assay and the PCR-based N-ras assay.
  • The TDT assay successfully detected strand breaks during induced apoptosis.

Conclusions:

  • The TDT assay provides a simple and semiquantitative tool for studying DNA strand breaks resulting from DNA damage.
  • This method offers a more sensitive and efficient alternative for assessing DNA integrity.