Related Experiment Videos
Tenascin suppresses CD3-mediated T cell activation
1Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Bunkyo-ku, 113-8602, Japan.
Biochemical and Biophysical Research Communications
|September 15, 1998
Summary
Tenascin (TN) is an immunosuppressive extracellular matrix protein that inhibits T cell proliferation. It interferes with T cell activation regardless of the costimulatory molecules used.
Area of Science:
- Immunology
- Cell Biology
- Extracellular Matrix Biology
Background:
- Tenascin (TN) is an extracellular matrix protein known to antagonize fibronectin (FN) actions.
- TN interferes with FN-dependent cell attachment and activation.
Purpose of the Study:
- To investigate the inhibitory effect of TN on T cell proliferation.
- To determine if TN affects T cell activation induced by anti-CD3 antibody with various costimulators in serum-free conditions.
Main Methods:
- Human T cells were cultured in serum-free conditions.
- T cell proliferation was induced using immobilized anti-CD3 antibody combined with costimulators like FN, VLA-4/FN, VLA-5/FN, VLA-6/laminin, LFA-1, CD2, and CD28.
- Phorbol ester stimulation was used as a control.
Main Results:
- TN completely inhibited T cell activation induced by anti-CD3 plus FN.
- TN interfered with T cell proliferation costimulated by VLA integrin/ligand interactions (VLA-4/FN, VLA-5/FN, VLA-6/laminin).
- TN also inhibited T cell activation costimulated by LFA-1, CD2, and CD28, but not by phorbol ester.
Conclusions:
- Tenascin (TN) inhibits anti-CD3-induced T cell proliferation irrespective of the costimulatory molecules used.
- TN acts as a generally immunosuppressive extracellular matrix protein.
- TN potentially interferes with T cell receptor/CD3-mediated T cell activation.