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Related Experiment Videos

Carbon source utilisation by Bordetella bronchiseptica

B J Plotkin1, D A Bemis

  • 1Department of Microbiology, College of Osteopathic Medicine, Midwestern University, Downers Grove, IL, USA.

Journal of Medical Microbiology
|September 15, 1998
PubMed
Summary

Bordetella bronchiseptica utilizes various organic acids and amino acids for energy, but not carbohydrates. Carbon source impacts bacterial growth, motility, and adherence to host cells.

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Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Host-Pathogen Interactions

Background:

  • Bordetella bronchiseptica is an opportunistic pathogen affecting various animal hosts.
  • Understanding its metabolic capabilities is crucial for controlling infections.
  • Previous studies have indicated limited substrate utilization by B. bronchiseptica.

Purpose of the Study:

  • To comprehensively investigate the sole carbon and energy sources utilized by Bordetella bronchiseptica.
  • To confirm and extend previous findings on carbohydrate and sugar alcohol utilization.
  • To assess the impact of different carbon substrates on key bacterial characteristics.

Main Methods:

  • Culturing Bordetella bronchiseptica isolates representing different phase types and animal origins.

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  • Assessing utilization of tricarboxylic acid cycle intermediates, organic acids, amino acids, carbohydrates, and sugar alcohols as sole carbon/energy sources.
  • Measuring doubling time, piliation, flagellation, motility, capsule production, and adherence to mammalian cells.

    • Main Results:

    • B. bronchiseptica efficiently utilized succinate, citrate, alpha-ketoglutarate, fumarate, lactate, oxaloacetate, pyruvate, acetate, proline, glutamate, glutamine, and tyrosine.
    • The inability to utilize carbohydrates and sugar alcohols was confirmed and extended across various isolates.
    • Substrate availability influenced bacterial doubling time, motility, piliation, flagellation, capsule production, and adherence.

    Conclusions:

    • Bordetella bronchiseptica possesses a versatile metabolic profile, primarily relying on organic acids and amino acids.
    • Its limited ability to metabolize carbohydrates suggests specific nutritional requirements and potential host-specific adaptations.
    • The observed influence of carbon substrates on bacterial phenotypes highlights the importance of host metabolic environment in B. bronchiseptica pathogenesis.