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Reverse transcriptase-polymerase chain reaction study of anion exchanger-2 in canine tissues and different
G Prime1, S Ebner, M Marin-Grez
1Institute of Physiology, University of Munich, Germany. G.PRIME@lrz.uni-muenchen.de
Kidney International. Supplement
|September 15, 1998
Abstract:
Reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA from canine large intestine, skeletal muscle, pancreas, kidney, and spleen and from cultured wild-type and C7 and C11 Madin-Darby canine kidney (MDCK) cells revealed considerable variation in anion exchanger (AE)1 and AE2 mRNA levels between the tissues. Similar high levels of AE2 mRNA were detected in all the MDCK cell populations. AE2 in MDCK cells is probably the basolateral Cl-/HCO3- exchanger common to the principal and beta-intercalated cells.
