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[The evaluation of lymphocyte subpopulation by flow cytometry in healthy children]
A Iwaszkiewicz-Pawłowska1, A Stasiak-Barmuta, J Zak
1I Kliniki Chorób Dzieci Akademii Medycznej w Białymstoku.
Insights
This study on pediatric lymphocyte subpopulations found age-related changes in immune cells like CD19+ and CD2+ cells. These findings establish reference values for assessing children
Area of Science:
- Immunology
- Pediatrics
- Flow Cytometry
Context:
- Limited data exists on lymphocyte subpopulations in specific pediatric age groups.
- Understanding age-related immune system development is crucial for accurate health assessments.
- Flow cytometry is a key technique for analyzing immune cell populations.
Purpose:
- To investigate age-specific changes in lymphocyte subpopulations in children aged 2 months to 15 years.
- To establish reference values for lymphocyte subpopulations using flow cytometry in pediatric populations.
- To analyze trends in CD19+, CD2+, CD3+, CD4+, CD8+, CD56+, and Th/Ts ratios across different pediatric age ranges.
Summary:
- A study of 49 children using flow cytometry identified significant age-related shifts in lymphocyte populations.
- From age three onwards, a progressive decrease in CD19+ cells was observed, alongside increases in CD2+, CD8+, and CD56+ cells.
- The Th/Ts ratio also decreased with age, while CD3 and CD4 counts showed no significant age-related differences.
Impact:
- Provides essential reference values for lymphocyte subpopulations in pediatric peripheral blood.
- Aids in the accurate assessment of the immunological status of children.
- Highlights critical developmental changes in the pediatric immune system detectable by flow cytometry.
Abstract:
Seeing that amount of experiences concerning the cytometry of lymphocyte subpopulation in particular age ranges is very low we considered that undertaking this subject is useful. By the method of flow cytometry 49 children at the age from 2 months to 15 years were studied. According to the results of our studies we found, starting from the third year of life decrease of mean percentage values of cells CD19+, progressive in relation to the age, increase of mean percentage values of cells CD2+, increase of percentage values of cells CD8+ and cells CD56++ and the decrease of mean values of the indicator Th/Ts. Remaining CD3, CD4 did not show any statistically significant differences in investigated age ranges. Determined by the method of flow cytometry the percentage and complete values of lymphocyte subpopulations in peripheral blood in investigated age ranges can make the reference values in the assessment of immunological state.