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Related Experiment Videos

Problems with BHK 21 cells

F Brown1

  • 1Plum Island Animal Disease Center, Greenport, NY 11944, USA.

Developments in Biological Standardization
|September 16, 1998
PubMed
Summary
This summary is machine-generated.

Passaging baby hamster kidney (BHK 21) cells in suspension culture alters cell properties, affecting foot-and-mouth disease virus (FMDV) attachment and vaccine stability. This method may also select for antigenically different FMDV variants.

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Area of Science:

  • Cell Biology
  • Virology
  • Biotechnology

Background:

  • Baby hamster kidney (BHK 21) cells are commonly used for virus propagation.
  • Cell culture methods, such as suspension culture, can alter cellular properties.
  • Foot-and-mouth disease virus (FMDV) vaccine production relies on efficient virus growth and inactivation.

Purpose of the Study:

  • To investigate how passage in suspension culture modifies BHK 21 cell properties.
  • To assess the impact of suspension culture on FMDV attachment and virus characteristics.
  • To evaluate the implications for FMDV vaccine production.

Main Methods:

  • Culturing BHK 21 cells in suspension versus monolayer.
  • Analyzing surface expression of integrin chains involved in FMDV attachment.

Related Experiment Videos

  • Observing actin stress fiber formation.
  • Assessing FMDV particle stability after inactivation.
  • Characterizing FMDV antigenic properties after growth in different cell cultures.

    • Main Results:

    • Suspension-cultured BHK 21 cells showed down-regulation of alpha5 and alphaV integrin chains.
    • Loss of actin stress fibers was observed in suspended cells.
    • FMDV grown in suspended cells produced particles less stable to aziridine inactivation.
    • Growth in suspension culture led to selection of antigenically variant FMDV strains.

    Conclusions:

    • Passage in suspension culture significantly alters BHK 21 cell surface integrins and actin cytoskeleton.
    • These cellular changes impact FMDV attachment, stability, and antigenicity.
    • Current methods for FMDV vaccine production using suspension-cultured cells may require re-evaluation due to altered virus properties and potential for antigenic variation.