Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superoxide radical production by sponges Sycon sp

A V Peskin1, Y A Labas, A N Tikhonov

  • 1Institute of Developmental Biology, Russian Academy of Sciences, Moscow. peskin@ibrran.msk.su

FEBS Letters
|September 17, 1998
PubMed
Summary

Sea sponges (Sycon sp.) spontaneously generate superoxide radicals in seawater. This production, measured at 0.5 nmol/min per sponge, was inhibited by superoxide dismutase and restored by KCN.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mid-holocene Brown Bear (Ursus arctos) from the Bolshoy Lyakhovsky Island (New Siberian Islands).

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections·2023
Same author

The Interaction of Water-Soluble Nitroxide Radicals with Photosystem II.

Applied magnetic resonance·2021
Same author

Electron Transfer through the Acceptor Side of Photosystem I: Interaction with Exogenous Acceptors and Molecular Oxygen.

Biochemistry. Biokhimiia·2017
Same author

Corrigendum to "Assay of superoxide dismutase activity in a plate assay using WST-1" [Free Radic. Biol. Med. 103 (2017) 188-191].

Free radical biology & medicine·2017
Same author

A finding of the male mammoth carcass in the Karginsky suit of the Upper Pleistocene of the Taimyr Peninsula.

Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections·2015
Same author

Chlorophyll fluorescence induction, chlorophyll content, and chromaticity characteristics of leaves as indicators of photosynthetic apparatus senescence in arboreous plants.

Biochemistry. Biokhimiia·2014

Area of Science:

  • Marine Biology
  • Biochemistry
  • Environmental Science

Background:

  • Superoxide radicals (O2-.) are reactive oxygen species with significant biological and environmental implications.
  • Marine organisms, including sponges, may play a role in the biogeochemical cycling of reactive oxygen species in seawater.
  • Previous studies suggested abiotic production of superoxide in seawater, but biological sources required further investigation.

Purpose of the Study:

  • To investigate the spontaneous production of superoxide radicals by marine sponges (Sycon sp.) in seawater.
  • To quantify the rate of superoxide radical outflow from sponges into their surrounding environment.
  • To elucidate the enzymatic mechanisms and chemical factors influencing sponge-derived superoxide production.

Main Methods:

  • Utilized the catechol Tiron as a specific scavenger to detect and quantify superoxide radicals.
  • Measured the rate of superoxide radical outflow from individual Sycon sp. sponges in controlled seawater conditions.
  • Assessed the effect of copper-zinc superoxide dismutase (Cu,Zn-SOD) and potassium cyanide (KCN) on superoxide generation.

Main Results:

  • Sea sponges (Sycon sp.) were confirmed to produce superoxide radicals at a high rate in seawater without external stimuli.
  • The rate of superoxide radical outflow reached 0.5 nmol/min per sponge at pH 6.5.
  • Superoxide generation was significantly inhibited by Cu,Zn-superoxide dismutase and could be restored by the addition of KCN, indicating an enzymatic component.

Conclusions:

  • Marine sponges are a biological source of superoxide radicals in seawater.
  • The enzymatic machinery of sponges contributes to superoxide production, with potential implications for marine oxidative stress and biogeochemistry.
  • Findings support and extend previous observations of abiotic superoxide production in seawater, highlighting a dual abiotic-biotic source.

Related Experiment Videos