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Objective evaluation of Papanicolaou staining
Y Sakamoto1, E Taniguchi, K Kakudo
1Department of Pathology, Wakayama Medical College, Japan.
Analytical and Quantitative Cytology and Histology
|September 18, 1998
Abstract
Objective:
To evaluate the color of cytoplasm stained by Papanicolaou staining quantitatively.
Study Design:
Cytoplasm photographed under the same conditions was measured for spectral reflection by colorimetry, and the color of the cytoplasm was shown by the L*a*b* color specification.
Results:
This method enabled us to specify the color of cytoplasm, which was classified in light green, eosin and orangeophilic.
Conclusion:
The variation in the color of cytoplasm by different staining methods was quantified and related to the dyes absorbed by the cytoplasm.