Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Remote quantitation server for quality assurance in DNA ploidy analysis

G Haroske1, W Meyer, F Theissig

  • 1Institute of Pathology, University of Technology, Dresden, Germany. haroske@rcs.urz.tu-dresden.de

Analytical and Quantitative Cytology and Histology
|September 18, 1998
PubMed
Summary

Remote quality control for DNA ploidy analysis is feasible and comparable to local methods. This framework ensures reliable diagnostic interpretation and consistent high product quality in daily routines.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Document standards for pathology reports in digital medicine].

Der Pathologe·2020
Same author

Solitary plasmacytoma of thoracic vertebra in a woman with Lynch syndrome: A case report.

International journal of surgery case reports·2019
Same author

["Digital Pathology in Diagnostics-reporting on digital images" guideline of the Professional Association of German Pathologists].

Der Pathologe·2018
Same author

[Annual report 2017/18 of the working group on informatics, digital pathology and biobanks].

Der Pathologe·2018
Same author

Near-K-Edge Double and Triple Detachment of the F^{-} Negative Ion: Observation of Direct Two-Electron Ejection by a Single Photon.

Physical review letters·2018
Same author

["Digital Pathology in Diagnostics" guideline : Reporting on digital images].

Der Pathologe·2018

Area of Science:

  • Analytical Cellular Pathology
  • Biomedical Engineering
  • Quantitative Cytology

Background:

  • The 1997 European Society of Analytical Cellular Pathology consensus established guidelines for DNA image cytometry.
  • Implementing robust quality control is crucial for reliable diagnostic interpretation in cytometry.

Purpose of the Study:

  • To remotely test a stepwise quality control framework for DNA image cytometry.
  • To evaluate the EUROQUANT quantitation server for automated, server-based analysis.
  • To assess the feasibility and comparability of remote versus local DNA ploidy analysis.

Main Methods:

  • Utilized two series of measurements on breast cancer biopsies and rat liver imprints.
  • Performed measurements at two cytometry workstations, with data transferred to a central quantitation server.

Related Experiment Videos

  • Employed automated analysis including DNA histograms and error detection, with continuous monitoring of key parameters.

    • Main Results:

    • Demonstrated the preconditions and outcomes of server-based quality control in DNA ploidy analysis.
    • Confirmed that remote analysis is feasible and yields results comparable to local DNA ploidy analysis.
    • Showcased how quality control tests effectively reflect process performance at various levels.

    Conclusions:

    • Server-based quality control is a viable method for DNA ploidy analysis.
    • Remote quality control ensures reliable diagnostic interpretation and consistent product quality.
    • Quality control measures should be integrated into daily laboratory routines.