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Related Experiment Videos

'98 Escherichia coli SWISS-2DPAGE database update

L Tonella1, B J Walsh, J C Sanchez

  • 1Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland. luisa.tonella@dim.hcuge.ch

Electrophoresis
|September 18, 1998
PubMed
Summary

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The Escherichia coli SWISS-2DPAGE database was established using 2-D PAGE and protein identification techniques. This resource aids in understanding the E. coli proteome, accelerating future research.

Area of Science:

  • Proteomics
  • Molecular Biology
  • Biochemistry

Background:

  • The Escherichia coli SWISS-2DPAGE database is a crucial resource for proteomic research.
  • Advances in protein separation and identification techniques are essential for comprehensive proteome mapping.

Purpose of the Study:

  • To report recent progress in the development of the E. coli SWISS-2DPAGE database.
  • To detail the methodologies employed for protein separation and identification within the E. coli proteome.

Main Methods:

  • Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) with immobilized pH gradients and SDS-PAGE.
  • Protein identification using amino acid composition, N-terminal microsequencing, Edman sequence tag, and gel comparison.
  • Electroblotting onto polyvinylidene difluoride membranes and amido black staining for spot visualization.

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Main Results:

  • Established the E. coli SWISS-2DPAGE database, accessible via the ExPASy molecular biology server.
  • Identified 231 polypeptides on the E. coli SWISS-2DPAGE map using various techniques.
  • Confirmed 65 out of 153 putatively identified proteins through gel comparison, with many identified by multiple methods.

Conclusions:

  • The E. coli SWISS-2DPAGE database is a valuable tool for proteome analysis.
  • Continued advancements in biochemical methodology and genome completion will accelerate E. coli proteome research.