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A new technic for counting Schistosoma japonicum eggs in pig feces
A L Willingham1, M V Johansen, E H Barnes
1Danish Center For Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark. awi@kvl.dk
Abstract:
An improved laboratory method was developed for counting Schistosoma japonicum eggs in pig feces, which involves filtration, sedimentation and centrifugation, but avoids toxic chemicals. It is sensitive, allows easy differentiation from similar-sized and shaped protozoan cysts, and permits evaluation of egg viability both by direct viewing of eggs and miracidial hatching. It was found to be significantly better at recovering eggs than the modified Bell filtration technic. The sensitivity, specificity and practicality of this technic make it our method of choice for studies on porcine schistosomiasis japonica.