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Biochemical separations by continuous-bed chromatography

T L Tisch1, R Frost, J L Liao

  • 1Bio-Rad Laboratories, BioMaterials Division, Hercules, CA 94547, USA. ted_tisch@bio-rad.com

Journal of Chromatography. A
|September 19, 1998
PubMed
Summary
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Continuous-bed technology offers advanced polymeric supports for biomolecule purification. This chromatography innovation enables high-resolution separations at high flow-rates without compromising binding capacity.

Area of Science:

  • Biochemistry
  • Chromatography
  • Materials Science

Background:

  • Traditional column-packing media for biomolecule purification evolved from polysaccharide beads to polymeric supports.
  • Continuous-bed technology represents a novel approach utilizing in-situ polymerization within the chromatographic column.

Purpose of the Study:

  • To introduce and evaluate continuous-bed technology as an advanced support for biomolecule purification.
  • To demonstrate the properties and advantages of continuous-bed supports for chromatographic separations.

Main Methods:

  • Polymerization of advanced monomers and ionomers directly within a chromatographic column.
  • Formation of a homogeneous network of interconnected microparticle nodules with large interstitial voids.
  • Characterization of the nonporous nodule surface and large overall surface area.

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Main Results:

  • Continuous-bed supports create a dense, homogeneous network with high hydrodynamic flow due to large channels.
  • The nonporous yet high-surface-area nature of the polymeric microparticles ensures high binding capacity.
  • High-resolution biomolecule separations were achieved at high flow-rates.

Conclusions:

  • Continuous-bed technology provides a significant advancement in chromatography media for biomolecule purification.
  • This innovative support system allows for efficient, high-capacity, and high-resolution separations.
  • The technology offers a promising alternative to traditional packing materials, enhancing chromatographic performance.