Calcium imaging: a primer for mycologists

This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development are highly dependent upon Ca2+. The methodologies for imaging Ca2+ fluxes continue to develop rapidly. These methodologies are explained here in a style that should be accessible to a newcomer to the field, hopefully forming a bridge to the more complex methodological literature.