Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease of normotest/thrombotest discrepancy in non-contacted coumarin plasma after glass or ellagic acid activation

A Girolami, G Patrassi, A Burul

    Blut
    |July 1, 1976
    PubMed
    Summary

    Coumarin activation shortens Thrombotest clotting times, reducing the Normotest/Thrombotest discrepancy. This indicates Thrombotest sensitivity to contact phase and factor VII activation, not coumarin inhibitors.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High incidence of anti-FVIII antibodies against non-coagulant epitopes in haemophilia A patients: a possible role for the half-life of transfused FVIII.

    British journal of haematology·1996
    Same author

    Antiphospholipid antibodies, recurrent thromboembolism, and intensity of warfarin anticoagulation.

    Thrombosis and haemostasis·1996
    Same author

    Type I factor XIII deficiency is caused by a genetic defect of its b subunit: insertion of triplet AAC in exon III leads to premature termination in the second Sushi domain.

    Blood·1996
    Same author

    The risk of abortion and stillbirth in antithrombin-, protein C-, and protein S-deficient women.

    Thrombosis and haemostasis·1996
    Same author

    "Pseudo homozygous" activated protein C resistance due to double heterozygous factor V defects (factor V Leiden mutation and type I quantitative factor V defect) associated with thrombosis: report of two cases belonging to two unrelated kindreds.

    Thrombosis and haemostasis·1996
    Same author

    Prothrombin fragment 1+2 and thrombin-antithrombin complex levels in patients with inherited APC resistance due to factor V Leiden mutation.

    British journal of haematology·1996

    Area of Science:

    • Hematology
    • Clinical Chemistry
    • Coagulation Studies

    Background:

    • Coumarin anticoagulants are widely used, necessitating reliable monitoring methods.
    • Discrepancies between different clotting assays can complicate patient management.
    • Understanding assay sensitivity to coagulation factors is crucial for accurate interpretation.

    Purpose of the Study:

    • To investigate the effect of coumarin plasma activation on Thrombotest and Normotest clotting times.
    • To elucidate the underlying mechanisms responsible for observed clotting time discrepancies.

    Main Methods:

    • Activation of coumarin plasma in glass tubes for 60 minutes.
    • Measurement of Thrombotest (TT) and Normotest (NT) clotting times in undiluted and diluted plasma.
    • Comparison of clotting times before and after activation, and after ellagic acid activation.

    Related Experiment Videos

    Main Results:

    • Coumarin plasma activation significantly shortened Thrombotest clotting times.
    • Normotest clotting times showed a lesser degree of shortening.
    • The NT/TT discrepancy decreased progressively after coumarin plasma activation.
    • Similar, though less pronounced, effects were observed after ellagic acid activation.

    Conclusions:

    • Thrombotest is highly sensitive to contact phase activation and factor VII activation.
    • The observed shortening of clotting times is attributed to activation of these pathways, not coumarin-induced inhibitors.
    • These findings have implications for interpreting coagulation test results in patients on coumarin therapy.