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Related Experiment Videos

CD95 expression in aged mice infected with tuberculosis

A D Roberts1, I M Orme

  • 1Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.

Infection and Immunity
|September 24, 1998
PubMed
Summary
This summary is machine-generated.

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This study found that CD95 (also known as Fas receptor) is not poorly expressed on T cells in old mice infected with Mycobacterium tuberculosis. Instead, CD95 expression levels were similar or higher in older mice, contrary to previous hypotheses.

Area of Science:

  • Immunology
  • Cell Biology
  • Aging Research

Background:

  • The CD95/ligand pathway is crucial for T cell apoptosis and immune homeostasis.
  • Evidence suggested this pathway may be impaired in aged individuals.
  • T cell function and apoptosis regulation are critical during aging and infection.

Purpose of the Study:

  • To investigate CD95 expression on T cells in aged mice during Mycobacterium tuberculosis infection.
  • To determine if CD95 expression is reduced in T cells of old mice.
  • To analyze the phenotype of CD95-expressing T cells in aged, infected mice.

Main Methods:

  • Flow cytometry analysis of T cells from young and old mice infected with Mycobacterium tuberculosis.
  • Quantification and intensity measurement of CD95 expression on T cells.

Related Experiment Videos

  • Assessment of CD44 and CD45RB expression patterns on T cells.

    • Main Results:

    • CD95 expression was not reduced on CD4 T cells from old mice; numbers were similar to young mice.
    • CD95 expression intensity was potentially higher on T cells from old mice.
    • CD44 and CD45RB expression changes mirrored those in young mice, with CD95(hi) cells showing an activated phenotype (CD44(hi) CD45RBhi).

    Conclusions:

    • The study refutes the hypothesis of poor CD95 expression on T cells in aged mice during M. tuberculosis infection.
    • CD95 expression and T cell activation markers appear to be maintained in older mice.
    • These findings suggest the CD95 pathway's role in T cell homeostasis may not be significantly compromised by aging in this model.