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Related Experiment Videos

Ribonucleoprotein particles of quiescent maize embryonic axes

A Rincón-Guzmán1, E Beltrán-Peña, A Ortíz-López

  • 1Departamento de Bioquímica, Conjunto E, Facultad de Química, Universidad Nacional Autónoma de México, México, DF.

Plant Molecular Biology
|September 25, 1998
PubMed
Summary

Researchers isolated ribonucleoprotein particles (RNPs) from maize seeds, identifying 7S RNP and signal recognition particles (SRP). These RNPs contain specific RNAs and associated proteins, offering insights into RNA storage in quiescent cells.

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Area of Science:

  • Molecular Biology
  • Plant Science
  • Biochemistry

Background:

  • RNA molecules are often stored in ribonucleoprotein particles (RNPs) during metabolic quiescence.
  • RNPs play crucial roles in gene regulation and cellular processes.

Purpose of the Study:

  • To isolate and characterize RNPs from embryonic axes of mature maize seeds.
  • To identify the specific RNA and protein components of these RNPs.

Main Methods:

  • Sucrose and CsCl gradient centrifugation for RNP isolation.
  • Northern blot analysis using specific probes for RNA identification.
  • Protein content characterization.

Main Results:

  • Two non-ribosomal RNP types were identified: 7S RNP and signal recognition particle (SRP).

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  • 7S RNP contains 5S ribosomal RNA (rRNA) and a TFIIIA-homologous protein.
  • SRP contains 7S RNA and associated GTPase proteins (p72, p68, p54).

    • Conclusions:

    • Maize seed RNPs, including 7S RNP and SRP, are composed of distinct RNA and protein complexes.
    • These findings contribute to understanding RNA storage mechanisms in plant seeds.