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Updated: Aug 13, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
[Hypochromic effect of signal attenuation in 31P-NMR spectra of linear polyphosphates]
V I Krupianko1, V M Vagabov, L V Trilisenko
1Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow oblast, Russia.
Abstract:
Chemical shifts in 31P-NMR spectra of linear polyphosphates were studied. In each polyphosphate species tested, the sum of signal intensities of the internal (core) phosphate groups was proportional to the concentration of each polyphosphate, but the contribution of such groups to the total intensity of the signal decreased with increasing the length of the polyphosphate chain. An equation for estimating the polyphosphate chain length in biological objects taking into account a decrease in the 31P-NMR spectral intensity is proposed.
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