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Related Experiment Videos

Isolation, characterisation and mapping of simple sequence repeat loci in potato

D Milbourne1, R C Meyer, A J Collins

  • 1Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee, UK.

Molecular & General Genetics : MGG
|September 28, 1998
PubMed
Summary

New simple sequence repeat (SSR) markers were developed for potato (Solanum tuberosum L.) genetic studies. These SSR markers offer high polymorphism and are recommended to replace Restriction Fragment Length Polymorphisms (RFLP) for future research.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Simple sequence repeats (SSRs) are valuable genetic markers in various species.
  • Potato (Solanum tuberosum L.) genetic research can benefit from robust and informative molecular markers.

Purpose of the Study:

  • To develop and characterize novel SSR markers for Solanum tuberosum L.
  • To assess the polymorphism and utility of these SSR markers for genetic mapping in potato.

Main Methods:

  • Extraction of Solanum tuberosum L. DNA sequences with SSR motifs from databases and libraries.
  • Enrichment of DNA libraries using triplex affinity capture or single-strand hybridization selection.
  • Design and synthesis of 112 primer pairs, followed by amplification and polymorphism analysis in potato clones.

Related Experiment Videos

  • Segregation analysis to locate SSR loci on potato genetic linkage maps.

    • Main Results:

    • 112 primer pairs successfully amplified potato DNA products.
    • 98 primer pairs revealed length polymorphisms in diploid and tetraploid potato clones.
    • 89 loci from 65 primer pairs were mapped onto two potato genetic linkage maps.
    • 52 SSRs were identified as single-locus markers, with maps aligned using SSR and RFLP loci.

    Conclusions:

    • The developed SSR markers exhibit high information content and polymorphism in potato.
    • These SSR markers are suitable for genetic mapping and are proposed as replacements for RFLP markers in future potato research.