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Related Experiment Videos

Characterization of granular particles isolated from postsynaptic densities

S L Lai1, S C Ling, L H Kuo

  • 1Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.

Journal of Neurochemistry
|September 29, 1998
PubMed
Summary

Researchers isolated and characterized protein aggregates from pig brain

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • The postsynaptic density (PSD) is a crucial structure at neuronal synapses.
  • Understanding the molecular composition of the PSD is key to deciphering synaptic function.

Purpose of the Study:

  • To isolate and biochemically characterize protein aggregates from the postsynaptic density.
  • To identify the protein constituents and structural properties of these aggregates.
  • To correlate these findings with known structures within the PSD.

Main Methods:

  • Isolation of protein aggregates from pig cerebral cortex.
  • Biochemical analysis, including disulfide bond assessment.
  • Negative staining electron microscopy for morphological characterization.

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  • Immunoblotting to identify specific protein components.

    • Main Results:

    • Protein aggregates were isolated, primarily linked by disulfide bonds.
    • Electron microscopy revealed granular objects (~21 nm diameter) with surface protrusions.
    • Major constituents identified as tubulin and a 70 kDa protein.
    • Minor components included calcium/calmodulin-dependent protein kinase II and glutamate receptor subunits; actin was absent.
    • Biochemical and morphological data support identification with ~20 nm PSD granular particles.

    Conclusions:

    • The isolated granular protein aggregates represent a specific component of the postsynaptic density.
    • These findings provide insights into the molecular organization and potential function of PSD granular particles.
    • The study postulates the spatial arrangement of these aggregates within the postsynaptic terminal.