[HLA-Cw genotyping of serologically and non-serologically defined alleles using sequence-specific primers (PCR-SSP)
Abstract:
The new classical complement-mediated microlymphocytotoxicity test can detect a total of 10 different serologically defined antigen specificities (CWl-10) encoded by HLA-Cw locus. However, the blank amounts to 20%-50% for there are Cw antigens which can not be detected with antisera available. We adopted a PCR-SSP method to genotype a sample of 70 subjects collected from a Chinese Han population in Shanghai. We identified for the first time the following non-serologically defined HLA-Cw alleles in the Chinese Han population: Cw*1201/1202,1203,1301,14,1601,1602,1701, and also the serologically defined HLA-Cw alleles such as: Cw*01,02,0302/0304,0303,04,0501,0602,0701/0702/0703,08. With this sample we have made a survey of HLA-Cw allele frequencies in a Chinese Han population in Shanghai, with blank frequency being lowered from 0.307 to 0.028. The Cw*08 was originally considered to be rare in Chinese population. However, we found 12 cases of Cw*08 in this sample, which clearly does not coincide with the original point of view. Our results confirmed that PCR-SSP typing is rapid and accurate for HLA-Cw alleles.
