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Related Experiment Videos

Brain Ac39/physophilin: cloning, coexpression and colocalization with synaptophysin

M Carrión-Vázquez1, A M Fernández, J Chowen

  • 1Neural Plasticity Department, Instituto Cajal (CSIC), Madrid, Spain. carrion.mariano@mayo.edu

The European Journal of Neuroscience
|September 30, 1998
PubMed
Summary

Physophilin, identified as the Ac39 subunit of V-ATPase, interacts with synaptophysin. This interaction is anatomically supported, suggesting coordinated gene expression and a role in V-ATPase regulation.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Physophilin is an oligomeric protein that binds synaptophysin, forming a complex potentially involved in the exocytotic fusion pore.
  • Previous studies suggested physophilin might be the Ac39 subunit of the V-ATPase, found in a synaptosomal complex with synaptophysin and other V-ATPase components.

Purpose of the Study:

  • To clarify the identity of physophilin and investigate its relationship with the V-ATPase and synaptophysin.
  • To provide anatomical evidence for the in vivo interaction between physophilin/Ac39 and synaptophysin.
  • To explore potential coordinated gene expression and functional roles of this complex.

Main Methods:

  • Cloning of physophilin from mouse brain and RT-PCR cloning from bovine adrenal medulla.

Related Experiment Videos

  • In situ hybridization to determine mRNA distribution in rat brain.
  • Immunohistochemistry and double-labeling immunofluorescence to analyze protein localization and colocalization.

    • Main Results:

    • Sequencing confirmed physophilin is identical to the Ac39 subunit of V-ATPase, correcting previous discrepancies.
    • Ac39/physophilin mRNA shows a neuronal distribution correlating with synaptophysin and V-ATPase subunit c.
    • Ac39/physophilin protein localizes to the neuropil, colocalizing with V-ATPase subunit A and partially with synaptophysin.

    Conclusions:

    • The findings provide anatomical support for the in vivo interaction between synaptophysin and Ac39/physophilin.
    • Suggests coordinated transcription of V-ATPase and synaptophysin genes.
    • Proposes a potential role for Ac39/physophilin in V-ATPase inactivation via V1 sector disassembly.