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Updated: Aug 19, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Published on: August 5, 2008
Quantitative hybridization to genomic DNA fractionated by pulsed-field gel electrophoresis
1Laboratory of Developmental Genetics, Wadsworth Center, New York State Department of Health, Albany, NY, USA.
Abstract:
Hybridization to genomic DNA fractionated by CHEF electrophoresis can vary >100-fold if the DNA is acid depurinated prior to Southern blotting. The level of hybridization is high or low depending on whether the molecule being analyzed migrates at a size coincident with or different from the size of the majority of genomic DNA in the sample, respectively. Techniques that avoid acid depurination including in-gel hybridizations and UV irradiation of DNA prior to blotting provide more accurate quantitative results. CHEF analysis of DNA molecules containing repetitive satellite sequences is particularly prone to this effect.

