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Related Experiment Videos

Expression of RGD minus fibronectin that does not form extracellular matrix fibrils is sufficient to decrease tumor

A Urtreger1, F Porro, L Puricelli

  • 1Institute of Oncology Angel H. Roffo, University of Buenos Aires, Argentina.

International Journal of Cancer
|October 1, 1998
PubMed
Summary

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Fibronectin (FN) expression reduces cancer cell metastasis. Even without forming extracellular matrix (ECM) fibrils or having the RGD binding site, FN significantly decreased lung metastasis in mice.

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Fibronectin (FN) is an extracellular matrix (ECM) glycoprotein that influences cancer cell invasion and metastasis.
  • The LMM3 cell line, derived from a highly metastatic mouse mammary adenocarcinoma, lacks both protein and mRNA expression of FN.

Purpose of the Study:

  • To investigate the role of fibronectin (FN) in the metastatic process.
  • To determine if FN expression, even without ECM assembly or the RGD binding site, can reduce cancer cell metastatic potential.

Main Methods:

  • Stable transfection of LMM3 cells with wild-type and RGD-minus variants of human FN cDNA.
  • Assessment of recombinant FN secretion, ECM assembly, in vitro migration, and adhesion.
  • Evaluation of experimental and spontaneous lung metastasis in vivo.

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Main Results:

  • All transfected clones secreted recombinant FN but did not assemble it into the ECM.
  • FN-producing cells exhibited reduced in vitro migratory ability and increased adhesion.
  • Significant reduction in both experimental and spontaneous lung metastasis was observed in FN-expressing clones.

Conclusions:

  • Expression of fibronectin (FN), even variants lacking the RGD site and ECM assembly capacity, is sufficient to decrease cancer cell metastatic potential.
  • An RGD-independent mechanism may contribute to FN's anti-metastatic effects.