In vivo and in vitro induction of c-fos in avian exocrine salt gland cells

Osmotic stress in ducklings (Anas platyrhynchos) results in salt secretion and adaptive cell proliferation and differentiation in the nasal glands. We investigated whether osmotic stress in vivo or muscarinic ACh receptor activation in vitro changed the expression levels of the cellular protooncogene products Fos and Jun, which may play a role in the initiation of the adaptive processes. Using Fos- and Jun-specific polyclonal antisera in Western blot experiments, we demonstrated that Jun is constitutively expressed in nasal gland tissue, whereas Fos is not detectable in tissue from unstressed (naive) animals. Under conditions of osmotic stress imposed by replacing the drinking water of the animals with a 1% NaCl solution, Jun protein remains constant in nasal gland tissue, whereas Fos protein is transiently upregulated. Treatment of cultured nasal gland tissue with muscarinic agonists results in a transcriptionally regulated expression of Fos in an atropine-sensitive manner. Immunohistochemical experiments show that Fos accumulation occurs in the nuclei of the secretory cells. These results indicate that the activation of the c-fos gene induced by muscarinic ACh receptor-mediated signaling pathways may play an important role in the initiation of adaptive growth and differentiation processes in nasal glands of osmotically stressed ducklings.