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Apoptosis and bioprocess technology

R P Singh1, M al-Rubeai

  • 1School of Chemical Engineering, University of Birmingham, Edgbaston, UK.

Advances in Biochemical Engineering/Biotechnology
|October 2, 1998
PubMed
Summary

Optimizing biopharmaceutical production in animal cell cultures requires understanding apoptosis, or programmed cell death. Strategies to suppress apoptosis, triggered by nutrient deprivation, improve large-scale cell culture processes.

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Area of Science:

  • Biotechnology and Bioprocess Engineering
  • Cell Biology and Molecular Medicine

Background:

  • Biopharmaceutical production relies heavily on optimizing animal cell line cultures.
  • Apoptosis (programmed cell death) is a primary cause of cell loss in these cultures.
  • Understanding and controlling apoptosis is crucial for efficient bioprocess development.

Purpose of the Study:

  • To review studies demonstrating apoptosis induction in industrially relevant animal cell lines.
  • To discuss factors that trigger apoptosis during large-scale cell cultivation.
  • To explore methods for suppressing apoptosis and their application in bioprocess optimization.

Main Methods:

  • Literature review of studies on apoptosis in animal cell cultures.
  • Analysis of factors inducing apoptosis, including deprivation of amino acids, glucose, serum, and oxygen.
  • Examination of reported methods for apoptosis suppression in industrial cell cultures.

Main Results:

  • Apoptosis is frequently induced in industrially important animal cell lines during cultivation.
  • Deprivation of essential factors like amino acids, glucose, serum, and oxygen are potent apoptosis inducers.
  • Recent reports show successful suppression of apoptosis under these culture conditions.

Conclusions:

  • Knowledge of apoptosis induction and suppression can lead to novel solutions for large-scale biopharmaceutical production.
  • Further research into apoptosis mechanisms is vital for advancing bioprocess technology.
  • Targeting apoptosis pathways offers a promising strategy for enhancing cell culture yields and efficiency.

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