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Bar-shaped nuclear chromatin in conjunctival samples: with cytologic features and ultrastructural correlation
T K Kobayashi1, Y Ishida, C Kaneko
1Department of Cytopathology, Saiseikai Shiga Hospital, Imperial Gift Foundation, Inc., Japan.
Acta Cytologica
|October 2, 1998
Summary
Bar-shaped chromatin in conjunctival cells, observed via electron microscopy, appears as nuclear membrane formations. These structures resemble nuclear grooves found in thyroid papillary carcinoma.
Area of Science:
- Ophthalmology
- Cytology
- Electron Microscopy
Background:
- Conjunctival cytology is crucial for diagnosing ocular surface conditions.
- Nuclear morphology is a key diagnostic feature in various cytopathology studies.
- Thyroid papillary carcinoma exhibits characteristic nuclear grooves.
Purpose of the Study:
- To investigate the cytomorphologic characteristics of bar-shaped chromatin in conjunctival samples.
- To compare these features with nuclear grooves observed in thyroid papillary carcinoma.
Main Methods:
- Cytological examination of 10 conjunctival samples from five volunteers.
- Detailed analysis of bar-shaped chromatin using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
Main Results:
- SEM revealed bar-shaped chromatin as fissures or shallow cracks on the conjunctival cell surface.
- TEM showed bar-shaped chromatin within the nucleus, associated with the nuclear membrane, and in the cytoplasm, enclosed by membranes.
Conclusions:
- Bar-shaped chromatin in conjunctival cells appears to originate from the nuclear membrane.
- The observed morphology shares similarities with cytoplasmic invaginations seen in thyroid papillary carcinoma.