A simple quantitative method to determine short chain fatty acid levels in biological fluids

A simple method has been developed for quantitative acetic, propionic, isobutyric, butyric, isovaleric, valeric, isocaproic, and caproic acids in intestinal fluids, feces and blood. The method utilizes extraction with ether and gas chromatography. It is accurate over a wide range of SCFA concentrations and appears to be applicable to any biological fluid.