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E-test for susceptibility testing of Mycobacterium tuberculosis

J Hausdorfer1, E Sompek, F Allerberger

  • 1Institute of Hygiene, University of Innsbruck, Austria.

The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease
|October 2, 1998
PubMed
Summary

The E-test showed high agreement for drug susceptibility testing of Mycobacterium tuberculosis. However, it produced a significant rate of false resistance, making it unsuitable for clinical use.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Accurate drug susceptibility testing is crucial for effective tuberculosis chemotherapy.
  • Standard methods for testing Mycobacterium tuberculosis susceptibility include proportion and radiometric methods.

Purpose of the Study:

  • To evaluate the performance of E-test strips for Mycobacterium tuberculosis drug susceptibility testing.
  • To compare E-test results with established methods like the proportion method and Bactec radiometric system.

Main Methods:

  • Mycobacterium tuberculosis isolates were tested using E-test strips for isoniazid, rifampicin, ethambutol, and streptomycin.
  • Results were compared against the proportion method (Lowenstein-Jensen medium) and the Bactec radiometric system.

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Main Results:

  • The E-test showed 90.1% concordance with the proportion method for susceptibility results against four antimicrobial agents.
  • While the E-test demonstrated false resistance in eight strains compared to Bactec, it showed no false susceptibility.
  • Discrepancies included mono-resistance to ethambutol and isoniazid, and combined resistance to ethambutol and streptomycin.

Conclusions:

  • The E-test exhibits a substantial rate of false resistance for Mycobacterium tuberculosis drug susceptibility testing.
  • Due to the high rate of false resistance, the E-test is not currently recommended for clinical laboratory use.