Capillary density and leukocyte adhesion in hamsters with hereditary cardiomyopathy

The aim of this study was to characterize microvascular networks in cheek pouch of cardiomyopathic Syrian hamster (CM) (Bio 14.6), which is an interesting model of idiopathic cardiomyopathy and congestive heart failure. Microcirculation was visualized by fluorescence microscopy. Diameter and length of arterioles, classified according to centrifugal ordering scheme, were measured. A computational method was arranged to determine the density of arterioles and capillaries (total vessel length per unit area, cm-1), fractal dimension of capillaries, and the associated Voronoi tesselation. Furthermore, leukocyte adhesion to venules and arteriolar reactivity to drugs were studied. Increase in the number of terminal arterioles and capillary rarefication characterized CM microvasculature compared with that of age-matched controls (58 +/- 7 versus 25 +/- 5 cm-1, and 128 +/- 15 versus 240 +/- 10 cm-1, respectively). Fractal dimension of capillaries was reduced in CM compared with controls (1. 40 +/- 0.10 versus 1.85 +/- 0.09) and associated with increased avascular spaces, as shown by Voronoi tesselation results. Leukocyte adhesion to venules increased significantly in CM. In CM responsiveness of arterioles to nitric oxide inhibition and propranolol was slighter but more marked to norepinephrine and angiotensin II compared with that of control hamsters. In conclusion, the different geometry, increased leukocyte adhesion, and altered arterial responsiveness may contribute to flow disturbances in the microcirculation of CM hamsters.