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Updated: Oct 23, 2026

Identification of Protein Interacting Partners Using Tandem Affinity Purification
Published on: February 25, 2012
The Tat protein of equine infectious anemia virus (EIAV) activates cellular gene expression by read-through
R Rosin-Arbesfeld1, D Willbold, A Yaniv
1Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
Abstract:
The Tat protein of equine infectious anemia virus, EIAV, was shown to augment viral gene expression, presumably through interaction with the Tat responsive element, TAR. Recently, cell-free polyadenylation assays suggested that perturbation of the EIAV TAR secondary structure diminished polyadenylation efficiency. The present study indicates that the EIAV TAR regulates the efficiency of the 3'-end processing of viral RNA also in transfected cells. Moreover, our data suggest that the provision of the EIAV Tat protein in trans potentiates read-through transcription through the 3' viral long terminal repeat (3' LTR), thus suggesting activation of downstream-located cellular genes.
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