Purification, crystallization and preliminary crystallographic analysis of bovine cytosolic brain-type creatine
1University of Illinois at Chicago, Department of Biochemistry and Molecular Biology, A-312 College of Medicine West, 1819 West Polk Street, Chicago, IL 60612-7334, USA. aloew@uic.edu
Acta Crystallographica. Section D, Biological Crystallography
|October 3, 1998
Abstract:
Creatine kinase (E.C. 2.3.7.2) is an important enzyme in energy metabolism which catalyzes the reversible transfer of a phosphoryl group between phosphocreatine and ADP to give ATP. Large quantities of a brain-type creatine kinase have been isolated from bovine photoreceptor cells and crystals suitable for X-ray diffraction analysis have been obtained by hanging-drop vapor diffusion. Crystals grow as tetragonal bipyramids in space group P43212 with cell dimensions a = b = 96.49, c = 108.42 A and diffract to at least 2.7 A resolution.
