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CD24 expression on human keratinocytes

P Redondo1, J García-Foncillas, I Okroujnov

  • 1Department of Dermatology, University Clinic of Navarra, School of Medicine, Pamplona, Spain. predondo@unav.es

Experimental Dermatology
|October 3, 1998
PubMed
Summary
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Human keratinocytes (HKC) express CD24, a cell surface glycoprotein. Stimulating HKC with calcium, PMA, or IFN-gamma increases CD24 mRNA and protein levels, suggesting a role in skin cell signaling.

Area of Science:

  • Cell Biology
  • Immunology
  • Dermatology

Background:

  • CD24 (Nectadrin) is a cell surface glycoprotein found on various cells, including lymphocytes and carcinoma cells.
  • Its precise function is unclear, but it's implicated in cell adhesion and signaling.
  • CD24 is the human homolog of murine heat-stable antigen (HSA), known to provide co-stimulatory signals in T-cell activation.

Purpose of the Study:

  • To investigate the expression and regulation of CD24 in normal human keratinocytes (HKC).
  • To determine if CD24 expression in HKC is modulated by common stimuli like calcium, PMA, and IFN-gamma.

Main Methods:

  • Long-term cultures of normal human keratinocytes (HKC) were established from breast skin sections.
  • Cells were treated with varying concentrations of calcium, phorbol-12-myristate-13-acetate (PMA), or interferon-gamma (IFN-gamma) for 24-48 hours.

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  • CD24 mRNA and protein levels were quantified using RT-PCR and flow cytometry.

    • Main Results:

    • Normal human keratinocytes (HKC) express low basal levels of CD24.
    • Treatment with calcium, PMA, or IFN-gamma significantly increased both CD24 mRNA and protein expression in HKC.
    • This study provides the first report on CD24 expression and production in cultured HKC under basal and stimulated conditions.

    Conclusions:

    • Human keratinocytes express CD24, and its expression is upregulated by specific stimuli.
    • The findings suggest a potential role for CD24 in keratinocyte biology and response to external factors.
    • Further research is warranted to elucidate the biological and therapeutic significance of CD24 in skin cells.