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Substrate selectivity and biochemical properties of 4-hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli
J R Pollard1, D Rialland, T D Bugg
1Department of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.
Applied and Environmental Microbiology
|October 6, 1998
Abstract:
4-Hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli was identified as a class I aldolase. The enzyme was found to be highly selective for the acetaldehyde acceptor but would accept alpha-ketobutyric acid or phenylpyruvic acid in place of the pyruvic acid carbonyl donor.