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[Relationship between methylenetetrahydrofolate reductase gene polymorphism and coronary heart disease]
1Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 150086 P. R. China. biology@ems.hrbmu.edu.cn
Objective:
This study inquired into the relationship between methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and coronary heart disease.
Methods:
By polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), MTHFR 677C-->T mutation was detected in 79 healthy controls and 69 patients with coronary heart disease.
Results:
The frequency of MTHFR variant V677 of patients was significantly higher than that of healthy controls (P<0.01).
Conclusion:
This study demonstrated that MTHFR gene V677 mutation was probably one of the genetic risk factors of coronary heart disease and this provided a new basis for exploring the relevant pathogenesis.