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Related Experiment Videos

The green fluorescent protein

R Y Tsien1

  • 1Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0647, USA.

Annual Review of Biochemistry
|October 6, 1998
PubMed
Summary
This summary is machine-generated.

Green fluorescent protein (GFP) from jellyfish is a valuable tool in biochemistry and cell biology. Its structure and function enable applications in gene expression, protein targeting, and biosensors.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Green fluorescent protein (GFP) from Aequorea victoria has rapidly gained prominence in scientific research.
  • GFP's unique internal fluorophore generation is of significant scientific interest and practical value.

Purpose of the Study:

  • To explore the structure-function relationship of GFP.
  • To highlight GFP's established and emerging applications in biological research.

Main Methods:

  • High-resolution crystal structure determination of GFP.
  • Protein engineering and mutagenesis of GFP.

Main Results:

  • Structural insights into GFP's spectroscopic function.
  • Demonstration of GFP as a marker for gene expression and protein localization.

Related Experiment Videos

  • Development of engineered GFP variants for advanced applications.

    • Conclusions:

    • GFP's structure is key to its spectroscopic properties.
    • GFP is a versatile tool for visualizing biological processes.
    • Engineered GFP holds promise for developing novel biosensors and photochemical tools.