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Related Experiment Videos

Retinoid-X receptor signalling in the developing spinal cord

L Solomin1, C B Johansson, R H Zetterström

  • 1The Ludwig Institute for Cancer Research, Stockholm Branch, Sweden.

Nature
|October 6, 1998
PubMed
Summary

Retinoid signaling is vital for development. New transgenic mouse assays reveal that retinoid-X receptors (RXRs) are activated in the spinal cord, playing a key role in motor neuron maturation.

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Area of Science:

  • Developmental Biology
  • Molecular Endocrinology
  • Neuroscience

Background:

  • Retinoids regulate gene expression via nuclear hormone receptors, including retinoic acid receptors (RARs) and retinoid-X receptors (RXRs).
  • Assessing ligand-activated receptor distribution in vivo, especially for RXR, has been challenging.
  • RXR's role as an unliganded auxiliary protein is established, but its ligand-activated function in vivo remains unclear.

Purpose of the Study:

  • To develop a novel assay for detecting ligand-activated nuclear receptors in vivo.
  • To investigate the in vivo role of retinoid-X receptors (RXRs) in the developing spinal cord.
  • To elucidate the specific activation patterns of RXR and RAR during embryonic development.

Main Methods:

  • Development of a transgenic mouse assay using effector fusion proteins (Gal4-RXR or Gal4-RAR).

Related Experiment Videos

  • In situ detection of ligand-activated receptors via an inducible transgenic lacZ reporter gene.
  • Analysis of receptor activation patterns in the developing spinal cord.

    • Main Results:

    • The assay successfully detected ligand-activated effector proteins in vivo.
    • Specific receptor activation patterns were observed in the spinal cord.
    • Evidence suggests RXR activation is crucial for the maturation of limb-innervating motor neurons.

    Conclusions:

    • Retinoid-X receptors (RXRs) function as bona fide receptors in the developing spinal cord.
    • The developed assay provides a powerful tool for studying nuclear receptor activity in vivo.
    • RXR activation is critical for motor neuron development and maturation.