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Related Experiment Videos

Method development in pharmaceutical analysis employing capillary electrochromatography

P D Angus1, E Victorino, K M Payne

  • 1Department of Pharmaceutical Chemistry and The Center for Bioanalytical Research, The University of Kansas, Lawrence 66067, USA.

Electrophoresis
|October 7, 1998
PubMed
Summary
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Capillary electrochromatography (CEC) method development was optimized for polar neutral pharmaceutical compounds. Spherisorb ODS-1 columns provided excellent separation efficiency, demonstrating repeatable performance for method development.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) is a powerful separation technique.
  • Developing robust methods for pharmaceutical compounds is crucial.

Purpose of the Study:

  • To develop and optimize CEC methods for separating structurally related polar neutral pharmaceutical compounds.
  • To compare different stationary phases for initial performance in reversed-phase CEC.

Main Methods:

  • CEC separations were performed using capillaries packed with Spherisorb ODS-1, Hypersil phenyl, and Hypersil MOS (3 µm particles).
  • Method development involved optimizing organic modifier type and percentage, buffer concentration, voltage, and temperature.
  • Separations were conducted using an acidic mobile phase (pH 3.0).

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Main Results:

  • High separation efficiencies were achieved, ranging from 200,000 to 260,000 plates/m.
  • Spherisorb ODS-1 columns yielded reduced plate heights of 1.22.
  • Repeatable column-to-column separation performance was demonstrated.

Conclusions:

  • Spherisorb ODS-1 is a suitable stationary phase for initial CEC method development of polar neutral compounds.
  • Optimized CEC parameters lead to high-efficiency separations.
  • The developed methods show promise for pharmaceutical analysis.