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Chiral separation of alpha-amino acids by ligand-exchange capillary electrophoresis using
A Végvári1, M G Schmid, F Kilár
1Central Research Laboratory, University of Pécs, Medical School, Hungary.
Electrophoresis
|October 7, 1998
Abstract:
The direct chiral resolution of underivatized alpha-amino acids by capillary zone electrophoresis (CZE) based on the principle of ligand exchange is described. An N-(2-hydroxyoctyl)-L-4-hydroxyproline/Cu(II) complex was used as a chiral selector. Besides amino acids containing aromatic residues, the basic amino acid histidine was resolved. Baseline separations were obtained for all amino acids investigated. The influence of selector concentration, electrolyte composition and pH on the resolution was investigated. It was found that there is a correlation between pI of the amino acids and the optimal pH.