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The enzymic synthesis of beta-[32P]UDP-N-acetylglucosamine
J Heptinstall1, P J Ward, I C Hancock
1Microbiological Chemistry Research Laboratory, University of Newcastle upon Tyne, United Kingdom.
Analytical Biochemistry
|November 1, 1978
Abstract:
Cells of Micrococcus sp. 2102 incorporate inorganic [32P]phosphate from the medium into the sugar-phosphate polymer of the wall. Controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives N-acetylglucosamine 6-[32P]-phosphate which can be purified by ion-exchange chromatography and incubated with UTP in the presence of crude preparations of phosphoacetylglucosamine mutase from Neurospora crassa and UTP:N-acetylglucosamine 1-phosphate phosphotransferase from Bacillus licheniformis which act in concert to synthesise beta-[32P]UDP-N-acetylglucosamine.