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A new method for allantoin determination and its application in allantoin determination in Agrostemma githago L. Seed
M M Vrbaski1, B Grujić-Injac, D Gajić
1Institute for Biological Research, Faculty of Science, Belgrade, Yugoslavia.
Analytical Biochemistry
|November 1, 1978
Abstract:
We have established several optimal conditions for qualitative and quantitative allantoin determination by applying Ehrlich's reagent. The limit of detection for allantoin determination amounts to 5 x 10(-6) mM. Allantoin is determined quantitatively by measuring the absorbance at 440 nm (from 300 to 1000 micrograms/ml). The color of the complex becomes stable by standing for 10 min at room temperature. We have used these conditions for allantoin determination in Agrostemma githago seed.