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Quantitative Limulus lysate assay for endotoxin activity: aggregation of radioiodinated coagulogen monomers
1Department of Internal Medicine, University of Texas Health Science Center, Southwestern Medical School, Dallas 75235, USA.
Analytical Biochemistry
|December 1, 1978
Abstract:
This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentration. The method is more precise and requires less lysate than the previously described quantitative assays for endotoxin activity.