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Measurement of S-adenosyl-L-methionine levels by SP Sephadex chromatography
1Laboratory of Medicinal Chemistry and Biology, National Cancer Institute, Bethesda, Maryland 20014, USA.
Analytical Biochemistry
|December 1, 1978
Abstract:
Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]adenosyl-L-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl-3H]adenosyl-L-methionine from [methyl-3H]methionine and other metabolites was achieved by stepwise elution with varying concentrations of HCl.