Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors Associated With Antiretroviral Therapy Reinitiation in Medicaid Recipients With Human Immunodeficiency Virus.

The Journal of infectious diseases·2019
Same author

Corrigendum to "A sequential analysis of motivational interviewing technical skills and client responses" [Journal of Substance Abuse Treatment 92 (2018 Sep) 27-34].

Journal of substance abuse treatment·2019
Same author

ESPACOMP Medication Adherence Reporting Guidelines (EMERGE): a reactive-Delphi study protocol.

BMJ open·2017
Same author

A Comparison of Adherence Timeframes Using Missed Dose Items and Their Associations with Viral Load in Routine Clinical Care: Is Longer Better?

AIDS and behavior·2016
Same author

"If I don't use a condom … I would be stressed in my heart that I've done something wrong": Routine Prevention Messages Preclude Safer Conception Counseling for HIV-Infected Men and Women in South Africa.

AIDS and behavior·2015
Same author

Current management of relapsing-remitting multiple sclerosis.

Internal medicine journal·2014

Related Experiment Video

Updated: Jul 27, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

A new chromogenic substrate for angiotensin-converting enzyme

A V Persson1, S F Russo, I B Wilson

  • 1Department of Chemistry, University of Colorado, Boulder 80309, USA.

Analytical Biochemistry
|December 1, 1978
PubMed
Summary

This study introduces a novel substrate, para-nitrobenzyloxycarbonylglycyl-(S-4-nitrobenzo-2-oxa-1,3-diazole)-L-cysteinylglycine, for angiotensin-converting enzyme. The enzyme

More Related Videos

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy
09:51

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy

Published on: March 27, 2017

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
07:31

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

Related Experiment Videos

Last Updated: Jul 27, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
12:03

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy
09:51

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy

Published on: March 27, 2017

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
07:31

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

Area of Science:

  • Biochemistry
  • Enzymology
  • Chemical Biology

Background:

  • Angiotensin-converting enzyme (ACE) plays a crucial role in the renin-angiotensin system.
  • Development of specific substrates is essential for studying enzyme kinetics and inhibition.
  • Fluorescent probes offer sensitive detection methods in biochemical assays.

Purpose of the Study:

  • To synthesize and characterize a novel chromogenic substrate for angiotensin-converting enzyme.
  • To investigate the enzymatic hydrolysis mechanism and product formation.
  • To establish a sensitive assay for ACE activity using the new substrate.

Main Methods:

  • Synthesis of para-nitrobenzyloxycarbonylglycyl-(S-4-nitrobenzo-2-oxa-1,3-diazole)-L-cysteinylglycine [NO2ZGly(S-NBD)CysGly].
  • Enzymatic hydrolysis assay using purified angiotensin-converting enzyme.
  • Spectrophotometric analysis of reaction products and intermediates.
  • Reaction with sulfhydryl reagent 4,4'-dithiodipyridine.

Main Results:

  • NO2ZGly(S-NBD)CysGly was hydrolyzed by ACE, yielding an S-benzfurazan derivative.
  • An immediate intramolecular S-to-N shift formed an N-benzfurazan derivative.
  • Reaction with 4,4'-dithiodipyridine produced a mixed disulfide with strong absorption at 461 nm.
  • A maximum difference in molar absorptivity of 13,000 M-1 cm-1 was observed at 470 nm.

Conclusions:

  • NO2ZGly(S-NBD)CysGly is a sensitive substrate for angiotensin-converting enzyme.
  • The observed spectral changes allow for sensitive detection of ACE activity.
  • This substrate facilitates the study of ACE and the development of enzyme inhibitors.