Synaptic changes in the perineal motoneurons of aged male rats

Cholera toxin-horseradish peroxidase (CT-HRP) was injected into the bulbocavernosus muscles of young (2 months of age) and old (19-20 months of age) male rats, and animals were killed 2 days later. The spinal cords containing the spinal nucleus of the bulbocavernosus (SNB) were dissected, processed with a modified tetramethylbenzidine method for visualization of retrogradely transported CT-HRP, and examined ultrastructurally. Neuronal structures apposing the membranes of 120 CT-HRP-labeled SNB motoneurons were analyzed by measuring the percentage of somatic membranes covered by synaptic contacts, synaptoid contacts, and neuron-neuron contacts. Most of the neuronal structures in the young and old SNB motoneurons consisted of synaptic contacts. The mean percentage of somatic membranes covered by synapses in old rats was significantly smaller than that in young ones. Size and number of synaptic contacts per unit length of somatic membranes in old animals were also significantly reduced. Plasma levels of testosterone in old males were significantly smaller than those in young ones. These age-related changes in synaptic inputs to SNB motoneurons and plasma levels of androgen seem to correlate with aging of the SNB system.